Bio-Rad PROTEAN Plus Multi-Casting Chamber - Manual (Page 5)

Note: Make sure that the plates and separation sheets inside the multi-casting chamber are
seated at the bottom of the multi-casting chamber.
7. Seat the gasket firmly in the notch in the multi-casting chamber body.
8. With the thumb screws loosened, guide the sealing plate into place, being careful not to
disturb the stack. The inlet port should match the groove at the bottom of the multi-
casting chamber. Be careful not to block the inlet port with a separation sheet. Gradually
tighten the screws in a random fashion until the plate is tight.
9. Stand the multi-casting chamber upright on the benchtop, and place it on a level surface.
Do not tip the multi-casting chamber upside-down at this stage.
Note: It may be easier to connect the 6–8 cm piece of tubing (Section 2.2) onto the multi-
casting chamber while it is still laying flat on the benchtop, prior to standing it upright.
2.2 Inlet Port Preparation
Casting gels from the TOP
Cut a piece of Tygon tubing (3 mm I.D.) about 6–8 cm in length. Connect one end of the
tubing to the multi-casting chamber port by twisting the tubing side to side until it is on about
1 cm. Attach a stopcock to the other end of the tubing. Close the stopcock valve to prepare for
casting (section 3).
Casting from the BOTTOM
Cut a piece of Tygon tubing (3 mm I.D.) about 6–8 cm in length (a). Connect one end of the
tubing to the multi-casting chamber port by twisting the tubing side to side until it is on about
1 cm. Fit a stopcock to the other end of the tubing (b). Attach the luer connector to the
stopcock (c). Tubing from the gradient former attaches to the luer connector (d). The other end
of the gradient former tubing then is fitted with a luer connector (e), and this fits into a
stopcock (f). This stopcock attaches directly to the gradient former. Close all valves to prepare
for casting (Section 3).
Fig. 2. Tubing and fittings to connect the Multi-Casting Chamber to the Gradient Former.
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a
b
c
d
e
f