Bio-Rad Trans-Blot® SD Semi-Dry Transfer Cell - Manual (Page 11)

2. Place a pre-soaked sheet of extra thick filter paper onto the platinum anode. Roll a pipet
or test tube over the surface of the filter paper (like a rolling pin) to exclude all air
bubbles. If thick or thin filter paper is used, repeat with one or two more sheets of buffer-
soaked filter paper.
3. Place the pre-wetted blotting media on top of the filter paper. Roll out all air bubbles.
4. Carefully place the equilibrated gel on top of the transfer membrane, aligning the gel on
the center of the membrane. Transfer will be incomplete if any portion of the gel is
outside the blotting media. Roll out all air bubbles.
5. Place the other sheet of pre-soaked filter paper on top of the gel, carefully removing air
bubbles from between the gel and filter paper. If thick filter paper is used, place two sheets
on top of the gel, and remove bubbles from between each layer. If thin filter paper is used,
place three sheets on top of the gel, and remove bubbles from between each layer.
6. If more than one full-size gel is to be transferred, place a sheet of pre-soaked dialysis
membrane on top of the filter paper stack. Repeat the procedure from step 2. Up to four mini
gels can be transferred at the same time by placing them side-by-side on the anode platform.
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