Bio-Rad Trans-Blot® SD Semi-Dry Transfer Cell - Manual (Page 8)

Catalog
Number
Product Description
Total Protein Detection Kits
170-6512
Biotin-Blot Protein Detection Kit
170-6517 Enhanced Colloidal Gold Total Protein Detection Kit
Blotting Standards
161-0372
Precision Prestained Standards, 10–250 kD, 500 µl
161-0380 Precision Streptactin-HRP conjugate
161-0381 Precision Streptactin-AP conjugate
161-0305 Prestained SDS-PAGE Standards, Low range
161-0309 Prestained SDS-PAGE Standards, High range
161-0307 Biotinylated SDS-PAGE Standards Kit, Low range, HRP
161-0308 Biotinylated SDS-PAGE Standards Kit, Low range, AP
161-0312 Biotinylated SDS-PAGE Standards Kit, High range, HRP
161-0313 Biotinylated SDS-PAGE Standards Kit, High range, AP
161-0306 Biotinylated SDS-PAGE Standards, Low range, 250 µl
161-0311 Biotinylated SDS-PAGE Standards, High range, 250 µl
Premixed Electrophoresis and Blotting Buffers
161-0732
10X Tris/Glycine/SDS, 1 L
161-0755 10X Tris/Glycine/SDS, 6 x 1 L
161-0734 10X Tris/Glycine, 1 L
161-0757 10X Tris/Glycine, 6 x 1 L
161-0733 10X Tris/Boric Acid/EDTA, 1 L
161-0756 10X Tris/Boric Acid/EDTA, 6 x 1 L
161-0741 10X TBE Extended Range, 1 L
161-0758 10X TBE Extended Range, 6 x 1 L
Section 3
Safety Instructions
Read the entire manual before beginning electrophoretic transfers.
Electrophoretic transfer of proteins and nucleic acids is dependent on many factors.
Observe the following guidelines to avoid mishaps that may result in serious damage to the
instrument or injury to the operator.
1. Do not reverse polarity on this instrument. This will result in corrosion and rusting of
the stainless steel cathode. If this should occur, the stainless steel should be cleaned with
a mild abrasive cleaner to remove the rust.
2. Do not exceed 25 V with this instrument. This could damage the electrodes.
3. Do not adjust the pH of transfer buffers unless specifically indicated. Follow instruc-
tions carefully. Adjustment of pH of transfer buffers, when not indicated, will result in
increased buffer conductivity. This is manifested by a higher than expected initial current
output as shown by the power supply's current meter. Monitor buffer resistance with the
Model 200/2.0 power supply prior to each run to insure proper buffer conductivity.
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