Bio-Rad Bio-Dot® and Bio-Dot SF Microfiltration Apparatus - Manual (Page 3)

Table of Contents
Page
Section 1 Introduction ..................................................................................1
1.1 Specifications............................................................................................1
Section 2 Special Handling and Features ..................................................1
2.1 Autoclaving ..............................................................................................1
2.2 Chemical Stability ....................................................................................1
Section 3 Bio-Dot SF Assembly ..................................................................3
3.1 Assembly ..................................................................................................3
3.2 Helpful Hints..............................................................................................5
Section 4 Protein Slot Blotting ....................................................................6
4.1 General Recommendations ......................................................................6
4.2 Immunoassay Procedure ..........................................................................6
Section 5 DNA Slot Blotting ........................................................................8
Section 6 RNA Slot Blotting ........................................................................9
6.1 Alkaline RNA Denaturation and Fixation ..................................................9
6.2 Glyoxal RNA Denaturation and Fixation ..................................................9
Section 7 Hybridization Protocols for Nucleic Acids ..............................11
7.1 Probe Recommendations ......................................................................11
7.2 Hybridization Protocols for DNA or RNA Bound to
Nitrocellulose or Zeta-Probe
® Membrane ..............................................11
7.3 Hybridization Protocols for RNA Probes ................................................13
7.4 Probe Stripping and Rehybridization ......................................................14
Section 8 Solutions for Protein Applications ..........................................15
8.1 Solutions for Nitrocellulose Membrane ..................................................15
8.2 Solutions for Zeta-Probe Membrane ......................................................16
Section 9 Solutions for Nucleic Acid Applications ..................................17
Section 10 Troubleshooting Guide ..............................................................19
Section 11 References ..................................................................................22
Section 12 Ordering Information ................................................................24