Bio-Rad Bio-Dot® and Bio-Dot SF Microfiltration Apparatus - Manual (Page 5)

Section 1
Introduction
The Bio-Dot SF blotting apparatus has an evenly spaced, slot shaped sample template for easy
slot blot sample comparisons. Because the Bio-Dot SF apparatus focuses the applied samples in
a thin line instead of a circle, this slot format makes it easy to use a densitometer to quantitate
results. The Bio-Dot SF apparatus is provided as a complete unit, or as a modular addition to the
Bio-Dot microfiltration system. Conversion of the Bio-Dot SF apparatus to the
Bio-Dot blotting apparatus is accomplished by purchasing the Bio-Dot module, which provides
the 96-well sample template.
The Bio-Dot SF slot format sample template has 48 wells with dimensions of 7 mm x 0.75 mm. The
wells are arranged in 8 rows and 6 columns. Sample can be applied using a standard pipet or
with an 8-channel pipet. The material used in the construction of the Bio-Dot SF blotting
apparatus can withstand rigorous sterilization and cleanup procedures. The Bio-Dot SF
apparatus can be repeatedly autoclaved, and is resistant to many chemicals, including acids,
bases, and ethanol.
1.1 Specifications
Materials
Bio-Dot SF apparatus Molded polysulfone
Bio-Dot SF gasket Silicone rubber
Stopcock Polytetrafluoroethylene (PTFE)
Shipping weight 600 grams
Overall size 13 x 15 x 6 cm
Membrane size 12 x 9 cm sheet
Autoclaving 15 minutes at 250°F (121°C) with a 1 minute fast exhaust
Chemical compatibility The Bio-Dot SF apparatus can be used with 100% alcohol solutions
and concentrated alkali or acid solutions. It cannot be used with
aromatic or chlorinated hydrocarbons. (See Table 1)
1