Bio-Rad Model 111 Mini IEF Cell - Manual (Page 5)

Section 1
General Information
1.1 Introduction
Bio-Rad’s Model 111 Mini IEF Cell introduces a simple and innovative “inverted” gel format
for analytical isoelectric focusing applications. The gel is run upside-down, directly contacting the
electrodes, to eliminate the need for electrode buffers and wicks. There is no need for active
cooling during the focusing process. Condensation within the cell is not a problem because the gel
is run upside-down, and therefore, the condensate cannot fall onto the gel.
The compact Model 111 Mini IEF Cell contains two graphite electrodes with an inter-
electrode distance of 5 cm. The graphite electrodes are removable for easy cleaning. A maximum
applied voltage of 450 volts is sufficient to yield tight bands, with excellent separation, within 90
minutes. The Model 111 Mini IEF Cell makes use of ultra-thin 0.4 mm gels, which provide better
heat dissipation than thicker gels. Casting these gels is simple with the casting tray and gel
support film provided with the cell. Acrylamide and agarose gels are both cast using the same
casting tray.
1.2 Specifications
Construction
Outer chamber Fabricated acrylic
Cell lid Polycarbonate
Casting tray Fabricated acrylic
Electrodes High purity graphite, 0.95 cm diameter
Sample templates Polyvinyl chloride
Shipping weight 1.8 kg
Overall size 24.6 (l) x 11.4 (w) x 4.8 cm (h)
Casting tray 20.3 (l) x 13.9 (w) x 2.3 cm (h)
Gel size 125 (w) x 65 (l) x 0.4 mm (t)
Glass plate size 125 x 65 mm
Voltage/power limit 500 VDC / 5 W
Casting tray temperature limit 60 °C
Note: The Model 111 Mini IEF Cell is not compatible with acetone, chlorinated hydrocarbons
(i.e., chloroform), or aromatic hydrocarbons (i.e., toluene, benzene).
To clean the cell components use a mild detergent, such as Bio-Rad’s Cleaning Concentrate
(catalog number 161-0722) or ethanol.
1