Bio-Rad Model 111 Mini IEF Cell - Manual (Page 22)

7. Autoradiography, biological activity, group specific staining, and immunological techniques
are just a few examples of many detection techniques currently used. The typical IEF stain is
Coomassie brilliant blue R-250.
Stain
0.2% Coomassie brilliant blue R-250
28% isopropanol or ethanol
14% acetic acid
Filter the stain before use. The gel should be stained a minimum of 30 minutes at room
temperature.
8. Destaining the gels should take only 30 minutes at room temperature.
Destain Solution
28% isopropanol or ethanol
14% acetic acid
9. Air dry the gel plate.
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