Bio-Rad CHEF-DR® III Variable Angle System User Manual
Page 28
5.5 Separations of DNA Size Standards
1. Restriction fragments
Size Range:
0.2–23 kb
Agarose:
1.2% Molecular Biology Certified
Buffer:
0.5x TBE
Temperature:
14 °C
Switch Time:
0.1 second
Run Time:
3 hours
Angle:
120°
Voltage Gradient:
9 V/cm
2. 5 kb Ladder
Size Range:
5–75 kb
Agarose:
1.0% Molecular Biology Certified
Buffer:
0.5x TBE
Temperature:
14 °C
Switch Time:
1-6 seconds
Run Time:
11 hours
Angle:
120°
Voltage Gradient:
6 V/cm
3. Lambda Ladder
Size Range:
50–1,000 kb
Agarose:
1.0% Molecular Biology Certified
Buffer:
0.5x TBE
Temperature:
14 °C
Switch Time:
50-90 seconds
Run Time:
22 hours
Angle:
120°
Voltage Gradient:
6 V/cm
4. Saccharomyces cerevisiae
Size Range:
240
–
2,200 kb
Agarose:
1.0% Pulsed Field Certified
Buffer:
0.5x TBE
Temperature:
14 °C
Switch Time:
60
–
120 seconds
Run Time:
24 hours
Angle:
120°
Voltage Gradient:
6 V/cm
25