Bio-Rad Image Lab™ Software - Manual (Page 129)

Quantity Tools
User Guide | 129
Absolute Quantity Tab
Absolute quantification is used to quantify bands based on known standard bands
using a calibration curve.
To calculate the absolute quantities of the bands
1. Select the Absolute tab.
2. Click Select.
3. Select at least two standard (known) bands and assign quantity values. Small
A’s appear (shown below circled in red) near the bands you selected.