Bio-Rad Bio-Plex Pro™ Human Chemokine Assays - Manual (Page 2)

Table of Contents
Introduction 1
Principle 2
Kit Contents and Storage 4
Recommended Materials 5
Assay Workflow 6
lmportant Considerations 7
Detailed Instructions 7
1. Plan Plate Layout 8
2. Prepare Instrument 9
3. Prepare Wash Method 10
4. Prepare Wash Buffer 11
5. Prepare Standards and Controls 11
6. Prepare Samples 14
7. Prepare Coupled Beads 16
8. Run Assay 18
9. Read Plate 22
Troubleshooting Guide 29
Appendix: Non-Human Primate (NHP) Cross-Reactivity 34
Plate Layout Template 35
Calculation Worksheet 36
Safety Considerations 38
Legal Notices 38
Ordering Information 39