Bio-Rad Bio-Plex Pro™ Human Chemokine Assays User Manual
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a.
Click
Add Panel
in the Select Analytes toolbar. Enter a new
panel name. Select Bio-Plex Pro Assay Magnetic from the
assay dropdown list. If using Bio-Plex Manager version 5.0 or lower,
select MagPlex from the assay dropdown list.
b.
Click
Add. Enter the bead region number and name for the first
analyte. Click
Add Continue to repeat for each analyte in the
assay. Refer to the bead regions in parentheses ( ) listed on the
peel-off label provided with the standards.
For reference, bead regions are shown in Table 13.
c.
Click
Add when the last analyte has been added and click OK to
save the new panel.
d. Highlight analytes from the Available list (left) and move to the
Selected list (right) using the Add button. To move all analytes at
once, simply click Add All.
e. If some of the analytes need to be removed from the Selected
list, highlight them and select Remove. If desired, it is possible to
rename the panel by clicking Rename Panel and entering a new
panel name.
Table 13. Bead regions for the human chemokine panel.
Analyte
Bead Region
Analyte
Bead Region
Analyte
Bead Region
6Ckine/CCL21
12
IL-1b
39
MDC/CCL22
29
BCA-1/CXCL13
74
IL-2
38
MIF
35
CTACK/CCL27
72
IL-4
52
MIG/CXCL9
14
ENA-78/CXCL5
73
IL-6
19
MIP-1a/CCL3
55
Eotaxin/CCL11
43
IL-8/CXCL8
54
MIP-1δ/CCL15
66
Eotaxin-2/CCL24 30
IL-10
56
MIP-3a/CCL20
62
Eotaxin-3/CCL26 65
IL-16
27
MIP-3b/CCL19
76
Fractalkine/CX3CL1 77
IP-10/CXCL10 48
MPIF-1/CCL23
37
GCP-2/CXCL6
15
I-TAC/CXCL11 25
SCYB16/CXCL16 64
GM-CSF
34
MCP-1/CCL2
53
SDF-1a+b/CXCL12 22
Gro-a/CXCL1
61
MCP-2/CCL8
57
TARC/CCL17
67
Gro-b/CXCL2
78
MCP-3/CCL7
26
TECK/CCL25
46
I-309/CCL1
20
MCP-4/CCL13 28
TNF-a
36
IFN-g
21