Bio-Rad Affi-Gel Protein A Media - Manual (Page 5)

Kit Components
The Affi-Gel protein A MAPS II kit contains a sufficient quan-
tity of reagents to purify approximately 500 mg of mouse IgG
1
.
Affi-Gel protein A One bottle containing 5 ml of
agarose gel suspended in 0.05 M sodium
phosphate, pH 7.5, with 0.05%
NaN
3
.
Binding buffer One bottle (471 g) of buffer
solids. Reconstitution
volume = 1,500 ml.
Elution buffer One bottle (25 g) of buffer
solids. Reconstitution
volume = 1,100 ml.
Regeneration buffer One bottle containing 400 ml.
Caution: Contains methanol.
Do not mouth pipette.
Column 1 x 10 cm Econo-Column ®
chromatography column.
Additional Items Required
Test tubes General purpose tubes for frac-
tion collection are recommended.
pH meter A pH meter is required to check
the pH of the binding and elution
buffers after reconstitution.
Mixer Standard laboratory magnetic
stirrer and bar for buffer mixing.
Balance Standard laboratory scale for
weighing out buffer solids.
Filter 0.22 micron filter for buffer
preparation.
Instructions for Use
Binding Buffer Preparation
The binding buffer is supplied as a premixed, preweighed solid.
Reconstitution and filtration are required prior to use. Dissolve 31.4 g
binding buffer solids per 100 ml distilled, deionized water. (Use the full
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