Bio-Rad Affi-Gel Protein A Media - Manual (Page 8)

The flow rate will be greater than or equal to 0.6 ml/min for a
1 ml column with an ID of 1 cm and a 2:1 buffer to gel ratio. A
typical sample run will take 1.0 to 1.5 hours for a one ml col-
umn.
4. Purification of IgG other than mouse with the Affi-Gel protein
A MAPS II kit:
Rabbit IgG from serum has been purified successfully with the
Affi-Gel protein A MAPS II kit (Bio-Radiations No. 53). The
protocol in this booklet can be used without modification.
5. Shelf life of the Affi-Gel protein A MAPS II kit:
The shelf life of Affi-Gel protein A agarose is 1 year at 4 °C. The
unreconstituted buffers are good for at least 1 year when stored
at room temperature.
References
1. Ey, P. L., Prowse, S. J. and Jenkin, C. R., Immunochemistry, 15, 429 (1978).
2. Bigbee, W. L., Vanderlaan, M., Fong, S. S. N. and Jensen, R. H. Mol. Immunol., 20, 153
(1983).
3. Kronvall, G. and Williams, R. C., J. Immunol., 103, 828 (1969).
4. Lindmark, R., Thoren-Tolling, K. and Sjoquist, J., J. Immunol. Methods, 62, 1 (1983).
5. Kronvall, G., Grey, H. M. and Williams, R. C., J. Immunol., 105, 1116 (1970).
6. Mackenzie, M. R., Warner, N. L. and Mitchell, G. F., J. Immunol., 120, 1493 (1978).
7. Chalon, M. P., Mine, R. W. and Vaerman, J. -P., Scand. J. Immunol., 9, 359 (1979).
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