Bio-Rad Affi-Gel 15 Gel - Manual (Page 10)

Fig. 4. Effect of amount of protein added on protein cou-
pling to Affi-Gel 10 gel.
A similar coupling capacity is
observed with Affi-Gel 15 gel.
mg protein coupled per ml gel
Conditions:
human gamma
globulin added
as shown
0.1 M MOPS, pH 7.5
10
20
30
40
10 20 30 40 50
mg protein added per ml gel
15
Fig. 3. Effect of time on protein coupling to Affi-Gel 10 gel.
A similar relationship is observed with Affi-Gel 15 gel.
% protein coupled
Conditions:
15 mg human
gamma globulin
added per ml of gel
0.1 M HEPES, pH 8.0
4 °C
10
20
30
40
50
60
70
80
10 20 30 40 50 60
Time (minutes)
14
LIT156B 6/17/98 10:41 AM Page 14