Bio-Rad Affi-Gel 15 Gel - Manual (Page 3)

Section 1
Introduction
Affi-Gel 10 and Affi-Gel 15 affinity supports are acti-
vated immunoaffinity supports that offer rapid, high effi-
ciency coupling for all ligands with a primary amino
group, including proteins throughout the entire range of
pIs and low molecular weight compounds such as pep-
tides.
1 Both Affi-Gel 10 and 15 supports are N-hydroxy-
succinimide esters of a derivatized crosslinked agarose gel
bead support, and both couple to ligands spontaneously in
aqueous or non-aqueous solution.
The Affi-Gel 10 support, which contains a neutral 10-
atom spacer arm, has been used to couple a variety of
materials in affinity chromatography, immunoadsorption,
and other techniques. The Affi-Gel 15 support contains a
cationic charge in its 15-atom spacer arm which signifi-
cantly enhances coupling efficiency for acidic proteins at
1
Section 9 Immunoaffinity Chromatography
with Affinity Supports ..................... 26
9.1 Adsorption of the Sample.......................... 26
9.2 Removal of Unbound Solutes.................... 29
9.3 Elution Strategies ...................................... 30
9.4 Special Considerations for Labile Antigens 34
9.5 Renaturation of Eluted Proteins ................ 35
Section 10 Ordering Information ..................... 36
Section 11 References......................................... 38
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