Bio-Rad Media Sampler Pack User Manual
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Section 1
Introduction
Macro-Prep ion exchange media are designed to provide high resolution and high
capacity for preparative separations. The rigid methacrylate beads exhibit little
shrinkage and swelling, making them suitable for both low- and medium-pressure
chromatography. The macroporous media allow both small and large molecules to
access exchange sites located throughout the chromatography bed. The physical
structure of the media permits high flow rates at low backpressure.
Bio-Rad's chromatography media are manufactured in an ISO 9001 registered
manufacturing facility. Manufacturing processes are audited and registered by
National Quality Assurance Limited under the provisions of ANSI/ISO/ASQ
9001:2000. Bio-Rad Laboratories guarantees quality of product in unopened
containers for 1 year from date of shipment. Regulatory support files are available
upon request for Macro-Prep ion exchange chromatography media. If you need
assistance validating the use of Macro-Prep media in a production process,
contact your local Bio-Rad Process Chromatography representative.
Section 2
Intended Use
Macro-Prep ion exchange media are appropriate for capture, intermediate, and
polishing purification steps. Due to their rigidity and unique surface chemistry,
these media are particularly suited for flow-through operations requiring high
throughput and high recovery of target molecules. The slightly hydrophobic base
bead also provides selectivity for removal of trace contaminants such as residual
host cell proteins, endotoxins, and viruses.
Macro-Prep media offer many advantages in both laboratory and process-scale
applications. They are easy to use, allow a quick and reliable packing, and can be
used for multiple cycles.
For maximum flexibility, the product offering includes Macro-Prep High Q strong
anion exchange support, Macro-Prep DEAE weak anion exchange support,
Macro-Prep High S strong cation exchange support, and Macro-Prep CM weak
cation exchange support.
All Macro-Prep ion exchange media contain carboxyl groups. Depending on the
media, pH conditions, and samples, the media can act in mixed mode. This
property can be exploited to give unique selectivity, which may increase yield and
purity.
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