Bio-Rad Media Sampler Pack - Manual (Page 5)

3
400 300 200 100 0
0
10
20
30
40
Linear flow rate, cm/h
Operating pr
essur
e, PSI
0.0
1.0
2.0
Operating pr
essur
e, bar
Fig. 1. Linear flow rate vs. operating pressure. Data for Macro-Prep High Q support was generated
on a 14 cm (ID) X 17.4 cm (H) Amicon Moduline glass column with 1/4” plumbing. A Cole-Parmer Masterflex
pump, size 18 neoprene tubing, Easy-Load pumphead was used. The eluent was water.
Table 1. Properties of Macro-Prep Ion Exchange Supports.
Macro-Prep High Q Macro-Prep DEAE Macro-Prep High S Macro-Prep CM
Support Support Support Support
Type of ion exchanger Strong anion Weak anion Strong cation Weak cation
Functional ligand –N
+
(CH
3
)
3
–N
+
(C
2
H
5
)
2
–SO
3
–
–COO
–
Ionic capacity 400 ± 75 µeq/ml 175 ± 75 µeq/ml 160 ± 40 µeq/ml 210 ± 40 µeq/ml
Dynamic binding capacity ≥37 mg BSA/ml ≥30 mg BSA/ml ≥49 mg IgG*/ml ≥25 mg Hemoglobin/ml
Counterion Cl
–
Cl
–
Na
+
Na
+
Nominal particle size† 50 µm 50 µm 50 µm 50 µm
Nominal pore size 1,000 Е 1,000 Е 1,000 Е 1,000 Е
Recommended maximum 3,000 cm/hr 3,000 cm/hr 3,000 cm/hr 3,000 cm/hr
linear flow rate
Chemical stability
1% SDS, 24 hr Yes Yes Yes Yes
6 M Guanidine-HCl, 24 hr Yes Yes Yes Yes
pH stability** 1–10 1–10 1–12 1–12
Volume changes
pH 4–10 <1% <1% <3% <1%
0.1–1.0 M NaCl <5% <5% <9% <4%
Autoclavable (121°C, 30 min) Yes †† Yes †† Yes Yes
Antimicrobial agent 20% ethanol 20% ethanol 20% ethanol 20% ethanol
* Human IgG
**Based on leachables, as determined by TOC (see Table 2)
† Based on underivatized beads
†† Do not autoclave in OH– form
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