Bio-Rad Bio-Rex® 5 Anion Exchange Resins - Manual (Page 6)

separation. Particle size will determine the flow rate,
which will affect the separation. The resin should be in
the correct ionic form and equilibrated prior to adding
the sample.
1. Calculate the amount of resin required based on the
expected resin capacity and sample concentration. If
the ionic concentration of the sample is unknown,
begin with 5 grams of resin for 100 ml of sample,
and optimize the volumes after obtaining the results.
2. Insure that the resin is in the proper ionic form,
which will allow the sample ions to be exchanged
onto the resin. If conversion of the resin to another
ionic form is necessary, use the guidelines in Table 2
for resin conversion.
3. Prepare the initial buffer, so that the pH and ionic
concentration will allow the sample ions to be
exchanged onto the column. For unknown solutions,
use deionized water.
4. Slurry and pour the resin into the column.
Equilibrate the resin in the initial buffer using 3 bed
volumes of buffer. Poorly equilibrated resin will not
give reproducible results.
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Instructions for Use
AG 3-X4, AG 4-X4, and Bio-Rex 5 resin may be
used in the batch method or the column method. The
batch method consists of adding the resin directly to the
sample and stirring. The column method requires
preparing a column filled with resin, and passing the
sample through.
Batch Method Instructions
The batch method is performed by adding the resin
directly into the sample and stirring. The resin should be
in the correct ionic form prior to beginning.
1. Weigh out about 5 grams of resin for every 100 ml
of sample. For larger scale applications or when an
exact amount of resin is needed, calculate the resin
volume based on the resin capacity.
2. Add resin to the sample and stir or shake gently for 1
hour.
3. Filter or decant the sample from the resin.
Column Method Instructions
The column method involves pouring a column with
the resin and passing the sample through to achieve the
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