Bio-Rad Bio-Rex® 5 Anion Exchange Resins - Manual (Page 8)

3. Neutral fraction.
a. Collect in a clean vial (scintillation vials work
well) and use a syringe to hasten flow through
the column.
b. Add the sample to column, drain through.
c. Wash with water (1 ml, 4 ml, 4 ml).
d. Cap and mix well. Store at 4 °C.
4. Acid fraction.
a. Change to a fresh vial.
b. Carefully add 1 ml of 10% sulfuric acid.
c. Wash with water (1 ml, 4 ml, 4 ml).
d. Cap and mix well.
Technical Information
If you have any questions or need additional
information, contact your local Bio-Rad representative.
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Since some of these compounds co-elute during HPLC,
this simple fractionation technique was developed.
Protocol
1. In a test tube, carefully pipet:
1.00 ml wine or grape must
0.100 ml 5% formic acid
0.100 ml 5% propylene glycol
0.2 ml 5 N ammonium hydroxide
Mix well. Insure that the pH is between 8 and 9;
adjust if necessary. Keep closed.
2. Prepare the column as follows (do not let the column
dry out):
a. Slurry 1 g Bio-Rex 5 100-200 mesh resin (Cl -
form) in 3 ml water.
b. Pour slurry into a polypropylene column.
c. Using a syringe, expel excess liquid, then wash
bed with 5 ml water.
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