Bio-Rad Bio-Gel A Agarose Gel User Manual

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Section 1
Introduction

Bio-Gel A gels are a series of agarose based size

exclusion gels which provide high resolving power,
minimal non-specific interaction, and excellent flow
properties. The porosity of the gel is controlled by the
percentage of agarose incorporated into the matrix. Six
fractionation ranges are available, with exclusion limits of
0.5, 1.5, 5, 15, and 50 million daltons.

Bio-Gel A gels are compatible with all commonly

used aqueous buffers. The gel has excellent shrink/swell
properties and exhibits no significant change in the bed
volume with increasing salt concentration up to 500 mM
salt. The gels can be run between pH 4–13 and can be
used at temperatures between 2–30 °C.

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