Bio-Rad Bio-Gel A Agarose Gel - Manual (Page 4)

Table 2. Properties of Bio-Gel A Gels
Product Hydrated Bead Typical Flow Fractionation
Size Rates* Range/Exclusion
Grade (µm) (cm/hr) Limit (daltons)
Bio-Gel A-0.5m gel
151-0130 Coarse 150–300 20–25 <10,000–500,000
151-0140 Medium 75–150 15–20 <10,000–500,000
151-0150 Fine 38–75 7–13 <10,000–500,000
Bio-Gel A-1.5m gel
151-0430 Coarse 150–300 20–25 <10,000–1,500,000
151-0440 Medium 75–150 15–20 <10,000–1,500,000
151-0450 Fine 38–75 7–13 <10,000–1,500,000
Bio-Gel A-5m gel
151-0730 Coarse 150–300 20–25 10,000–5,000,000
151-0740 Medium 75–150 15–20 10,000–5,000,000
151-0750 Fine 38–75 7–13 10,000–5,000,000
Bio-Gel A-15m gel
151-1030 Coarse 150–300 20–25 40,000–15,000,000
151-1040 Medium 75–150 15–20 40,000–15,000,000
151-1050 Fine 38–75 7–13 40,000–15,000,000
Bio-Gel A-50m gel
151-1330 Coarse 15–300 20–25 100,000–50,000,000
151-1340 Fine 75–150 5–15 100,000–50,000,000
* Flow rate determined by using a 1.5 x 20 cm column and a head to bed
ratio of 1:1.
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Section 2
Technical Description
Table 1. Bio-Gel A Gel Product Description
Matrix Bio-Gel agarose gel
Particle size range
Coarse 150–300 µm 50–100 mesh
Medium 75–150 µm 100–200 mesh
Fine 38–75 µm 200–400 mesh
Shipping medium Fully hydrated in water, with 0.05%
sodium azide added as a preservative.
Resistance
pH range 4–13
Maximum operating pressure 15 psi
Organic solvents 20% alcohol
Temperature range* 2–30 °C
Storage 4 °C to room temperature
* Note: Above this temperature range the gel softens and freezing causes
irreversible collapse of the gel structure and alteration of the exclusion
limit.
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