Bio-Rad MicroRotofor™ Cell - Manual (Page 4)

• Vacuum source and vacuum trap. (Vacuum in 22–28 mm Hg range)
• Deionized water
• Pipettes (100 µl – 2.75 ml volumes)
• Beaker or equivalent to hold the 3 ml sample volume.
Section 4
Setup and Operation
Note: For a detailed description of the MicroRotofor™ components, the
setup, and analysis of the results, please refer to the MicroRotofor™
instruction manual.
4.1 Overview
• Prepare the Focusing assembly: Electrode chambers and focusing
chamber with electrode membranes.
• Prepare and load the starter kit protein sample and seal the loading
ports.
• Add electrolyte solutions to the electrode chambers.
• Position the focusing assembly in the chassis and start the IEF run.
• Stop the run, remove the lid, and connect the system to a vacuum
source.
• Remove the loading port sealing tape and position the focusing chamber
into the harvesting station.
• Aspirate the fractions into the harvesting tray.
4.2 Prepare the Focusing Assembly
The focusing assembly consists of the electrode assemblies, the electrode
membranes and the focusing chamber.
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