Bio-Rad MicroRotofor™ Cell - Manual (Page 7)

1. Using the assembly tool as a template, cut two pieces of sealing tape
(Figure 4). Position the tape across the template covering all three
cutting grooves. Cut the tape with a cutting blade at all three grooves.
Each strip of tape can be picked up through the cutouts between two
grooves.
Fig. 4. Using the assembly tool to measure the appropriate length of sealing tape.
2. Seal the lower row of ports (harvesting ports) with a strip of tape. Make
sure to cover all of the ports, and to not extend the tape beyond the
focusing chamber (Figure 5).
Note: Excess tape, or tape not properly positioned, interferes with the
cooling block during oscillation, which can result in leakage of sample from
the focusing chamber.
Fig. 5. Applying sealing tape to cover the harvesting ports on the focusing chamber.
3. Fill the 3 ml syringe with sample. Slowly load the sample through the
centermost loading port of the focusing chamber (Figure 6). As this
channel fills, the sample will slowly spread to and fill all of the adjacent
channels. Continue adding sample slowly to this channel until all of the
channels are filled. Alternatively, fill every other channel and wait for the
sample to spread to the adjacent chambers.
Note: If air bubbles are introduced into the focusing chamber, the sample will
not spread to adjacent channels as described. Make sure to dislodge and
remove all air bubbles from the chamber by gently tapping or aspirating the
sample from a channel and loading it again.
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