Iris Sample Processing StatSpin® CenSlide 2000 Urine Sediment Analysis System - Manual (Page 16)

Special Topics
OPERATOR’S MANUAL
CenSlide 2000 Urinalysis System Page 3 - 2
5. Place the cap on the CenSlide tube and push down firmly
until it clicks and/or its rim is flush with the top of the
CenSlide tube. Invert two to three times to evenly mix
the specimen with the stain.
6. Refer to Chapter 2, pages 2-3 through 2-5 for instruction
on completing the preparation and reading of CenSlide
tubes.
3.0.2 Staining Characteristics
Red Blood Cells: In acid or neutral urine, the red cells
often remain intact and colorless or
stain slightly purple. In alkaline urine,
the red cells are usually lysed and
alkaline hematin forms, which stains
dark purple.
White Blood Cells: The nuclei of ordinary leukocytes
usually stain a dark orange-purple,
and the granularity of the cytoplasm
is distinguishable. There is variability
between light and dark forms.
Glitter Cells: These are large polymorphonuclear
leukocytes, frequently seen in
pyelonephritis, that appear pale
light blue to almost colorless. The
cells are larger than dark-staining cells.
Cytoplasmic granules with or without
Brownian movement may be noted.
Renal Epithelial The nucleus stains dark purple with
Cells: small rim of orange-purple cytoplasm.
Warning: This product will stain fabrics.
Use caution when dispensing.