Labconco Dry Ice Benchtop Freeze Dry System 7522700 - Manual (Page 3)

Instruction Sheet 7523200, Rev A C639 3 of 6
4. Samples may be pre-frozen by placing them in a freezer or by rotating them in the cold
solvent in the center chamber.
5. Turn on the vacuum pump.
6. When the vacuum is below 133 x 10 -3 mBar, samples may be added to the sample valve.
7. When the system is ready to begin lyophilization, attach the container with pre-frozen
sample to the valve. Apply vacuum to the sample by rotating the knob to the vacuum
position. (The bevel on the knob should be positioned toward the sample port). See
Figure 3.
Figure 3
8. When a sample is added to the system, the vacuum will decay. It is important to allow
the system vacuum to recover to 133 x 10 -3 mBar before additional samples are added to
the system.
9. Periodically check the quantity of dry ice in the center chamber and replenish as
necessary.
10. Let samples run until primary and secondary freeze-drying is complete. Release vacuum
by turning the valve to the vent position. The vent port may also be used to introduce
sterile air or dry Nitrogen into the sample container.
11. When lyophilization is complete, break the vacuum in the system by turning a manifold
valve to the vacuum position and immediately turn off the vacuum pump.
12. Allow all the dry ice to sublime and the system to warm up.
13. Remove the inner chamber and then drain the condensate from the outer chamber. Rinse
and dry all components.