Luminex FLEXMAP 3D Hardware User Manual - Manual (Page 50)

Clogs
Often, a clog somewhere in the Luminex ® FLEXMAP 3D ® instrument is the cause of a fluid
leak, a pressurization or sample probe problem, or a problem with calibration, verification, or
data acquisition.
To determine whether there is a clog, check the bead count during calibration. During
calibration you should see 300 bead events per second or more. If there are fewer than that,
there is probably a clog in the instrument.
In the event you encounter a problem that is clog-related, use the following procedure.
To troubleshoot a possible clog:
1. Clean and adjust the sample probe. See the section titled “ Cleaning the Sample Probe ”.
2. Perform the procedure for removing clogs. See the section titled “ Removing Clogs ”.
3. Replace all fluids.
4. Use the software to run the weekly maintenance routine.
5. Run calibration and verification.
If this procedure is unsuccessful, call Luminex Technical Support.
Pressurization Problems
Normal air and sheath pressure readings vary between 8 and 13 psi while the compressor
runs. If the instrument pressure is out of range, sample acquisition either can fail or can
return inadequate results.
TABLE 3. Pressurization Problems
Symptom Possible cause Solution
Pressurization fails or
pressure is too low. The sheath and waste lines are
not fully connected. Check the lines between the
sheath and waste bottles and the
Luminex FLEXMAP 3D
instrument.
The sheath or waste fittings are
cracked. Inspect the fittings to be sure they
form a tight seal. If not, call
Technical Support.
There is a leak in the instrument. Check for leaks. A leak is obvious
if there is fluid on the surface
where the FLEXMAP 3D
instrument sits. See the section
titled “
Fluid Leaks ”.
The compressor does not engage. Use the software to run a prime
command. If you do not hear the
compressor turn on, contact
Technical Support.
Troubleshooting
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