Multichannel Systems PH01 Manual - Manual (Page 6)

PH01 Manual
4
2 Installation and Operation
2.1 Welcome to the PH01 — Perfusion Cannula with Heating
Element and Sensor
Cell lines and tissue slices are placed and cultivated directly in the MEA culture chamber, on the
microelectrode array. All MEA systems can be upgraded with an enhanced perfusion system
(indicated by the code –E), featuring a perfusion cannula PH01 with programmable fluid
temperature and a two-channel temperature controller TC02 for controlling both the MEA
culture chamber temperature and the fluid temperature in parallel. A perfusion system is
required especially for recordings from acute slices.
The perfusion cannula with heating element and sensor PH01 and a temperature controller
TC01/TC02 is also useful for general perfusion purposes, whenever the fluid stream needs to be
temperature controlled.
The PH01 is to be used only for the temperature-control of non-corrosive liquids up to a
temperature of 50 °C. The programmable fluid temperature is controlled by a temperature
controller TC01/02. The PH01 is equipped with a standard platinum temperature sensor (PT
100).
Bath or test solutions can be applied at flow rates from 500 μl to 4.5 ml per minute. The
PH01 can be connected to any tubing that fits to the perfusion inlet (luer lock) and outlet
(1 mm OD stainless steel cannula). A titanium wire inserted into the cannula results in a
turbulent flow that ensures that the temperature of the liquid is constant across the flow
stream.
For setting up a complete perfusion system, a pump and a perfusion port for aspirating the
liquid from the culture chamber are required. Standard setups for MEA recording use a
peristaltic pump or syringe pump, and a standard metal cannula with beveled tip for
aspiration.
1 PH01
2 Perfusion inlet (luer
lock)
3 Perfusion outlet (1 mm
OD)
4 TC01/02 connector
5 Heating element cable
(connected to TC01/02)