Multichannel Systems PH01 Manual - Manual (Page 7)

PH01 Manual
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2.2 Setting Up and Connecting the PH01
Warning: Do not operate the PH01 unless it is completely filled with liquid. Otherwise,
overheating may irreversibly damage the PH01.
Warning: Do not start the perfusion until you have double-checked that the perfusion lines
are set up properly and that the inflow and outflow rate are matching. Spilled liquid may
irreversibly damage electronic instruments.
If the setpoint temperature is changed, the controller will need a short time to respond.
Please note that there will always be an intrinsic offset between the setpoint and the actual
temperature, at the tip of the cannula as well as when the liquid arrives in the culture
chamber. The offset at the tip depends mainly on the flow rate, see Flow Rate Dependent
Temperature Offset.
The temperature in the culture chamber depends on several parameters such as the tubing
diameter, length, and material (if a tube is used for guiding the liquid into the chamber), the
type of pump used, the flow rate, and so on. The user has to determine empirically the offset
of the individual setup once before starting the experiment.
The tube that leads into the perfusion chamber should be as short as possible to avoid that the
buffer cools down in the line. You can also carefully bend the metal cannula itself, so that the
outlet can be positioned directly in the chamber.
The flow rate should be constant during the experiment to ensure a stable temperature
control. Make sure that the ambient temperature is kept constant and shield the setup from air
drafts.
You can fix the PH01 directly onto the MEA1060 amplifier (or next to the perfusion chamber),
for example with tape.
1. Insert the into the perfusion inlet of the culture chamber (or carefully bend the cannula) so
that the liquid can flow into the culture chamber.
2. Connect the (luer lock) perfusion inlet of the to the perfusion tubing (connected to the pump)
and tightly secure it.
3. Connect one of the screws on the 's rear side to the ground of the amplifier/setup. Inside the
PH01, the cannula is connected to ground via a 10 μF capacitor. This reduces noise to a
minimum and avoids DC offset currents.
4. Make sure the temperature controller is switched off. Connect the to one channel of a
temperature controller TC01/02.
5. Fix the position of the perfusion cannula (for example, with tape).
6. Set up the aspiration system.