2 welcome to the usb-mea32-stim4 – Multichannel Systems USB-MEA32-STIM4 Manual User Manual

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USB-MEA32-STIM4 Manual

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1.2 Welcome to the USB-MEA32-STIM4

The USB-MEA32-STIM4 is a compact and portable stand-alone solution for extracellular
recordings with integrated amplifiers, data acquisition, and a four channel current stimulus
generator. Perfusion heating and the possibility to apply suction through perforated MEAs
are also included.

It is a highly compact system: The system acquires data from up to 32 electrode channels.
The digitized electrode data is transmitted to the connected computer via universal serial bus
(High Speed USB 2.0). Thus, it is possible to use any computer as a data acquisition computer,
also a laptop. Up to four systems can be controlled independently from a single computer.

Data is recorded, graphed, analyzed, and reviewed with the powerful and easy-to-use MC_Rack
program
from Multi Channel Systems MCS GmbH. You can export the data in standard formats
to other programs with the software MC_DataTool. MC_Stimulus II program controls the
stimulation on up to four of the twelve stimulation electrodes at a time. As a more convenient
alternative for LTP (long term potentiation) experiments, the new software package LTP-Director
and LTP-Analyzer is available. This software is able to control both, recording and stimulation
from one program, and also has many useful features for LTP recordings.

The USB-MEA32-STIM4 is designed primarily to do recordings from acute slices. Four different
layouts of the perforated MEAs are available, each with 32 recording and twelve separate
stimulation electrodes. The USB-MEA32-STIM4 is not compatible with the squarish standard
60 channel MEAs.

The integrated filter amplifier supports 32 electrode channels. The amplifier gain of 1000 is fixed.
The bandwidth of 0.5 Hz to 3 kHz is suitable for a broad range of applications, such as spikes and
field potential recordings.

The device can be used in combination with a binocular or an inverted microscope.

Acute slices can be kept in position and in good contact with the electrodes by applying suction
through the perforated MEA from below.

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