Checking the volume – BrandTech Scientific HandyStep S User Manual

Page 33

Advertising
background image

English

Checking the Volume

1.

Preparation of the instrument

Fitting tip. This test can be carried out with PD-Tips of any size.
However, size 5 ml is most commonly used for this purpose.

2.

Carry out the test

a) Adjust the stroke setting of HandyStep

®

S to Step 5 (10 % of

nominal volume).

b) Filling the PD-Tip, immersing it vertically into the testing liquid.
c) Discard the first step; it only serves to align the internal mecha-

nism.

d) Place the weighing vessel (containing a small amount of deionized

water) on a balance and tare the balance.

e) Dispense the second step into the weighing vessel. For this, push

down the dispensing lever at a steady rate to the stop and hold
it there. Then wipe the tip on the vessel wall over a distance of
approx. 10 mm.

f) Enter weighed value into test record.
g) Repeat points D to F 10 times.
(For the 10th volume test, the 5 ml PD-Tip with a stroke setting

of 5 has to be filled a second time – steps A through C.)

h) Repeat the same testing procedure at stroke settings 3 (6 % of

nominal volume) and 1 (2 % of nominal volume).

i) This results in a total of the 30 weighed values needed.

Depending on use, we recommend that gravimetric testing of the instru-
ment be carried out every 3-12 months. This time frame should be
adjusted to individual requirements. Gravimetric volume testing according
to DIN EN ISO 8655-5 is performed as follows:

33

Advertising