Next Advance 50-AU - Manual (Page 4)

4
Operator’s Manual for the Bullet Blender ® 50
minutes. To operate, press the “START”
button. After 1 minute, the LED light on
the number 1 will light up. After 2
minutes the second LED light by the
number 2 will light up. And so on. At the
setting shown above, after 12 minutes,
the Bullet Blender
® 50 will stop.
The BB50-AU model has a “4°C”
light that comes on when the internal
compartment of the instrument is
refrigerated. On the BB50-DX model, the
“Air Cooling™” light indicates that
ambient air is circulating through the
inside of the instrument during operation
and for 30 seconds after the cycle is
complete.
The BB50-AU unit contains a dry ice
cooling compartment. To use, fill the dry
ice cooling compartment with at least 2
lbs of dry ice. Always use cryogenic
gloves when handling dry ice. Do not use
the inner portion of the dry ice cooling
compartment to transport the ice. Do not
fill the dry ice cooling compartment with
water ice. Make sure that the dry ice
cooling compartment lid is properly
sealed. To pre-cool the instrument, after
filling the compartment with dry ice, run
the instrument for 5 minutes.
PROTOCOLS AND SAMPLE
SETTINGS
The following ratio should be used as
a guideline for determining the amount of
beads and buffer to use given a certain
sample size - 1 volume/mass of tissue : 1
volume of beads : 2 volumes of buffer.
For more specific information regarding the
use of various beads as well as specific
protocol information, please refer to our
website: www.nextadvance.com.
For 50mL tubes, do not operate with
more than 3.5g of sample, or a total of 20mL
of buffer, tissue, and beads (per tube).
Cutting the tissue into smaller pieces
will generally yield better results. Tissue
with a high aspect ratio (long, thin strips)
will homogenize better than tissue that is
round or cubic.
Do not operate the Bullet Blender ® 50
using the same tubes for more than 30
minutes.
Protocols for many types of samples are
posted on our website, at
www.nextadvance.com/FAQs/protocols.htm.
Notes:
Use Axygen ® brand or Corning ® brand
polypropylene, plug seal cap, skirted (self-
standing) 50 mL tubes. If there is a different
brand of 50 mL tube you would like to use,
please contact us at
[email protected]. There may be
some discoloration of the sleeves and of the
tubes after homogenization. This is normal
and a by product of the workings of the
Bullet Blender
® 50, which is designed to
impact the tubes with as much energy as
possible.
CLEANING
If you wish to clean your Bullet Blender,
To use Air cooling ™ , where ambient
air is drawn into the instrument, operate
the BB50-AU unit with the dry ice
compartment open. Air Cooling ™ is most
effective when the instrument is operated
in a 4°C environment.