Eppendorf Reference - Manual (Page 15)

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If the place where the pipette is used is at extremely high altitude, an adjustment
must be made in line with the ambient air pressure. At 1,000 m above sea level,
there is a volume error of approx. –0.4 %.
Assistance with these questions is contained in eppendorf‘s SOP (Standard
Operating Procedure). An SOP can be called up from our home page
www.eppendorf.com
If these checks prove to be unsuccessful, it is safe to assume that the
alignment of the pipette has altered (e.g. due to several components having
been replaced).
8.2.2 Follow-up alignment in the case of error
From a technical point of view, this is a zero-point shift. The value by which the
setting of the pipette is shifted remains constant across the entire measuring
range. If, for example, in the case of a 10 – 100 μL pipette, follow-up alignment
of 1 μL is carried out at 100 μL (=1 %), the pipette is also adjusted by 1 μL at
10 μL (= 10 %!)
Alignment Reference fix:
To assist you in finding the basic setting again, round adhesive labels with an
arrow are provided as an alignment aid.
Determine the volume by weighing and calculation
( see point a – c "Alignment Reference variable").
d
Pierce the calibration seal attached to the opening
for adjustments with Side B of the key provided.
This destroys the seal which should be removed.
Undo the screw inside a little until the control button
can be turned.
– Stick the alignment aid onto the control button.
C A L
Eppendorf Reference® – Part A – Chapter 8