Eppendorf Reference - Manual (Page 16)

15
e Adjust control button by the volume determined.
One revolution of the control button corresponds to the following values for
water:
Clockwise rotation: decrease in volume.
Counterclockwise rotation: increase in volume.
f Tighten the screw until the control button can no longer be turned.
Then continue as described in steps f and g of the Alignment Reference variable.
If the nominal volume does not correspond with the measuring result, repeat steps
d – g.
Then reseal the adjustment opening using one of the calibration seals supplied.
Alignment Reference variable:
a The pipette, tip and water must all be the same temperature
(20 – 25 °C, constant to ± 0.5 °C).
b Set the Reference variable to the desired nominal volume.
c With a pipette tip attached to the pipette, the desired volume is pipetted and
weighed 10 times. The average of this weighing is converted into μL using
the following formula:
The value obtained is the actual setting
(density of water at 20 °C: 0.9982 mg/μL; mg/μL = g/mL).
Reference fix Vol./revol.
1, 2, 5, 10 μL
10, 20 μL
25, 50 μL
100 μL
200, 250 μL
500, 1000 μL
1500, 2000, 2500 μL
approx. 0.5 μL
approx. 1 μL
approx. 2.4 μL
approx. 5 μL
approx. 12 μL
approx. 46 μL
approx. 118 μL
Volume =
Weight
Density of liquid
(at the temperature specified)
Eppendorf Reference® – Part A – Chapter 8