Leica Biosystems CM1860/CM1860 UV - Manual (Page 50)

50 Instructions for Use V1.4 – 10/2012
Trimming the specimen
Take care when handling microtome knives and disposable blades. The cutting edge is
extremely sharp and can cause serious injuries! For that reason, always wear the cut-
resistant safety gloves included in the standard scope of delivery!
• Insert the precooled knife/blade in the knife/blade holder.
• Adjust the appropriate clearance angle on the knife holder. Settings from 4° - 6° (knife holder
CN and blade holder CE-TC) or 1° - 2° (blade holder CE) are suitable for most applications.
• Align specimen with the knife holder/knife or blade holder/blade using the x-y orientation as
described on page 36.
• Remove the knife guard (knife holder CN) or fold the glass anti-roll guide (knife holder CE,
CE-TC) over to the side.
• Unlock the handwheel.
• To trim the specimen, move the specimen towards the knife using the coarse feed but-
tons. Trim the specimen down to the required sectioning plane by turning the handwheel.
( see 6.2.7 "Setting the section thickness" )
• Position the anti-roll guide on the knife and align with the cutting edge.
Adjust the anti-roll plate if necessary (
see "Clearance angle adjustment" ).
Attach the rod for the shelf to the inner front side
of the cryostat housing using the provided screws
(1) and the No. 3 Allen key, then attach the caps
(3). (The rear side of the movable shelf has white
plastic screws (2) that prevent the interior of the
chamber from being scratched.) Now hook the
movable shelf into the guide rod.
1
2
3 Fig. 51
7.3.6 Installing the shelf (movable) (optional)
7. Daily Use of the Instrument
Always clamp the specimen block BEFORE clamping the knife or the blade.
Lock the handwheel and cover the knife edge with the knife guard prior to any manipu-
lation of knife/blade or specimen, prior to changing the specimen block and during all
work breaks!