SciGene Hybex Microsample Incubator User Manual
Page 10
Hybex® Microsample Incubator — USER MANUAL
Version 1.2H, October 2014 9 (408) 733-7337, [email protected]
D. Calibrating the Temperature Controller
The temperature controller is calibrated at the factory to provide accurate block
temperatures from 35 to 99°C. Calibration is required only if the controller displays a block
temperature which differs by more than one degree (1°C) from a NIST calibrated T-type
digital thermometer (sold separately, SciGene cat. #1051-52-0). Contact SciGene Customer
Service (
) or your local distributor to purchase a thermometer.
To calibrate the temperature controller:
1.
Insert a block into the Hybex incubator, turn ON the
instrument and set the controller to 65°C. Allow 15
minutes for temperature to stabilize.
2.
Using the cable provided with the digital thermometer
(cat. #1051-52-0), plug one end into the blue receptacle
found on the back panel of the Hybex incubator and
the other end into the thermometer.
3.
Turn on the thermometer. Allow 1 minute for
thermometer to stabilize. The actual temperature of
the block will be displayed.
4.
Calculate the difference between the digital
thermometer and the temperature controller to
determine the adjustment value. For example, if the
thermometer reads 63.9°C while the controller reads
65°C, then the adjustment value is -1.1°C.
5.
On the controller, press the up and down arrows simultaneously for 3 seconds. The
left display shows “A1” and the right display shows “open”.
6.
Press the Advance Button (green circle) 3 times until the right display shows “i.CA”.
The left display will show the offset value between the controller and thermometer
when the unit was last calibrated.
7.
Using the up or down arrows, add the adjustment value from step 4 above to the
existing offset value shown on the controller. For example, if the adjustment value is
-1.1°C and the current offset is -0.3°C then the new offset is -1.4°C.
8.
Press the Infinity Key (∞) twice to exit calibra on and return to the opera on
display. Verify that the temperature on the thermometer matches the display. Your
incubator is now calibrated to provide accurate temperatures between 35 and 99°C.