SciGene Little Dipper Processor for FISH - Manual (Page 11)

Little Dipper ® Processor for FISH— USER MANUAL
March 2013 LD for FISH manual 2.0; software 1.4 for FISH 10
IV. SYSTEM OPERATION
A. Installing Baths and Centrifuge Buckets
1. Clean baths, racks and stir bars by rinsing in running tap water, followed by 100
ml of DI water and then 100 ml of ethanol (Fig. 10). Dry with a lint-free towel.
2. Clean sensors with DI water and a lint-free towel (Fig. 11).
3. Install standard or heatable low volume baths by rotating temperature sensors
up, placing baths and then lowering sensors (Fig. 12). For low volume baths
without temperature control, leave sensors down while placing baths.
4. Place a stir bar in all baths (Fig. 13). Low volume baths use low profile stir bars
(SciGene cat. #1080-11-1).
Fig. 10. Clean baths and racks. Fig. 11. Clean sensors.
Fig. 13. Add stir bars. Fig. 14. Unlock centrifuge.
Fig. 15. Insert buckets. Fig. 16. Insert pads and spacers.
5. Unlock and open the centrifuge (Fig. 14).
6. Hang a red bucket on the rotator arms tabs marked “B
ALANCE R ACK ”. Repeat
using a green bucket on the rotator arms marked “S AMPLE R ACK ” (Fig. 15).
7. Place an absorbent pad in each bucket with a black frame spacer on top (Fig. 16).
8. Balance the centrifuge by inserting a rack with the same number of slides as
the sample rack into the red bucket.
9. Close and lock the centrifuge.