SciGene 777 Microarray Oven - Manual (Page 8)

Model 777 Microarray Oven — USER MANUAL
Version 2.1, February 2011 (408) 733-7337, [email protected]
7
F. Calibrating the Oven Temperature
To ensure accurate (±0.5°C) oven temperatures, the performance of
the Watlow EZ-Zone controller should be periodically checked by
comparing the temperature displayed on the controller to an
accurate, NIST-certified (National Institute of Standards and
Technology) thermal measurement device. Calibration need only be
performed at a single temperature (e.g. 65°C) to achieve accurate
oven operating temperatures from ambient +5 to 99°C.
SciGene recommends the calibration method below to achieve
accurate oven temperatures. No other methods are recommended.
1. Calibration Check
a.
Turn on the oven, set to 65°C and allow temperature to
stabilize (30 min).
b. Connect a NIST-certified digital thermometer (SciGene Cat.
#1051-52-0) to the blue jack (secondary RTD) at the top back
of the oven.
c. Verify that the temperature shown on the digital thermometer
matches that shown on the controller within ±0.5°C. If it does
not, follow the steps below to adjust the controller.
2. Calibration Procedure
a.
Turn on the oven, set to 65°C and allow temperature to
stabilize (30 min).
b.
Connect a NIST-certified digital thermometer (SciGene Cat.
#1051-52-0) to the blue jack at the top back of the oven.
c.
Calculate the difference in temperature shown on the
controller to the digital thermometer. For example, if the
controller displays 65.0°C and the thermometer displays
66.5°C, the difference (offset) is 1.5°C.
d.
On the controller, press the up and down arrows
simultaneously for 3 seconds. The upper display will now read
“A1” and the lower display “open”.
e.
Press the green circle button 3 times until the lower display
shows “i.CA”. The upper display shows the offset value
between the controller and thermometer when the unit was
last calibrated.
f. Use the up/down arrows to adjust the offset value to the
temperature difference calculated in Step c. For example, if the
controller displays a temperature 1.5°C lower than the
thermometer, adjust the offset by adding 1.5 to the value shown.
g.
Press the ∞
key twice to return to the operation display. Your
oven is now calibrated to provide accurate temperatures
between ambient + 5 to 99°C.