UVP iBox Anesthesia System - Manual (Page 10)

iBox Anesthesia System 10
Figure 10: Adjusting the Vaporizer
Dial
Figure 9: Oxygen Flow Control
Panel
Breather
Control
Chamber
Control
Operating the iBox Anesthesia System
Prior to operation of the system, be sure that all components are properly connected as directed in
Setup of iBox Anesthesia System. Weigh charcoal filter canisters with each use. Replace the
charcoal filter after 100 grams of increased weight. The vaporizer should be in the off position and
the oxygen regulator dial turned completely off.
WARNING: A high isoflurane-to-oxygen ratio can result in harm or death to animals.
IMPORTANT: The following isoflurane flow and mix rates are a suggested starting point for
developing protocols (contact UVP for veterinarian field test of protocols). Parameters will vary from
lab to lab. All settings should be refined and adjusted based on careful observation of animals
during anesthesia.
NOTE: Proper oxygen flow rate is essential for correct use of the vaporizer. A higher oxygen flow
rate will lower the oxygen temperature and thereby reduce the production of isoflurane or
sevoflurane vapor. A very cool room may also negatively affect the production of vapor.
Setting the Oxygen Flow
WARNING: System MUST be properly set
up before turning on any gas.
Never disconnect any hoses until the oxygen and
vaporizer have been shut off. Failure to precisely follow
these instructions could result in injury. Back pressure can
cause vaporizer hoses to pop and cause isoflurane to
back up into the system, affecting system performance
and accuracy.
1. Using the Oxygen Flow Control Panel
(Figure 9), turn the Chamber Control
valve to the Chamber On position
and turn the Breather Control valve to Off.
2. Open the oxygen tank (Figure 1).
3. Turn the flow meter knob so that the flow meter reads
1 liter per minute (lpm).
4. Place animals into the induction chamber and close
the chamber lid.
5. For rapid induction, push down the white locking
mechanism (Figure 10) to move the vaporizer dial
(C). Turn the dial to 5 (%) for approximately one
minute, then reduce dial setting to 1.5 (%) for mice, 2
(%) for rats.
NOTE: The locking mechanism will only engage in
the OFF position.
6. Allow animals to remain in the induction chamber for
five minutes.
7. When animals are ready to be moved to the iBox
breathing device nosecone, turn the Breather Control
valve to Breather On. Then, turn the Chamber
Control valve to the Off position.
8. Turn the Chamber Control valve to the Chamber
Flush position for five seconds to clear the chamber
of isoflurane/sevoflurane. Then, turn the Chamber
Control valve to the Off position.