UVP iBox Anesthesia System - Manual (Page 11)

iBox Anesthesia System 11
9. Ensure that the warming plate is plugged into the Accessory outlet in the back of the iBox
Scientia and Spectra darkroom. The warming plate in the iBox Explorer is always on as long
as the system is on.
10. To turn the iBox warming plate on, flip the I/O (on/off) switch to the I (on) position. The
warming plate will show the temperature of the warming plate in the display.
11. Before transferring the animal to the breathing unit, check the surface of the warming plate for
proper temperature (37°C is recommended). The surface temperature of the iBox Explorer is
indicated on the warming controller box located in the back right corner of the darkroom (see
the iBox Explorer Imaging Microscope Instruction Guide for more information).
12. Increase the flow of gas at the flow meter to 3 lpm (going to the iBox).
13. Remove animals from the induction chamber and place on the warming plate. Remove the
black plug from the desired nosecones and place the animal’s nose into the nosecone. Be sure
there is a tight seal around the animal’s nose and that animal’s nostrils are not blocked by the
nose cone. Close observation of animal breathing and continued pink foot color helps
determine proper anesthesia level.
NOTE: Any nosecones not in use must be plugged with the black plugs (supplied).
Otherwise, gas will leak into the iBox darkroom.
14. Rats will remain anesthetized on the breathing device for up to twelve hours and mice for up to
six.
15. Once the procedure is finished, turn the Vaporizer Dial to the OFF position, remove the
animals from the nosecone and replace the black plug.
16. Turn the Chamber Control valve to the Chamber On position and allow oxygen to flow for
approximately two minutes to flush residual isoflurane/sevoflurane from the system.
17. If completely finished, close the oxygen tank and momentarily turn the Chamber Control valve
to the Chamber Flush position, allowing pressure from the tank to bleed off. Then, turn the
Chamber Control valve to the Off position
18. Turn the flow meter knob to the OFF position.
19. To perform another procedure, repeat the process from step one.