UVP iBox Anesthesia System - Manual (Page 12)

iBox Anesthesia System 12
Helpful Tips

Oxygen flow rates and isoflurane/sevoflurane concentrations in this manual are meant only as
guidelines. Operators should determine their own values based on observation and proper
training.

During longer procedures, it is important to continually monitor the animals in the iBox for
proper depth of anesthesia. It may be necessary to reduce the isoflurane/sevoflurane
concentration by 0.5 (%) during hours-long procedures.

If preparatory work is required before placing animals into the iBox, mice will remain
anesthetized for approximately one minute and rats for three to five minutes once removed
from the induction chamber.
Troubleshooting
Animals are waking up:

Ensure that the oxygen flow rate is adequate for the species being imaged.

Ensure that the vaporizer dial is turned to the appropriate isoflurane/sevoflurane
concentration.

Ensure that the quick disconnect fittings leading to the breather and chamber are
completely mated. The collar on the female metal fittings must be completely pushed
forward for complete mating to occur. Plastic fittings will give an audible ‘click’ when
properly mated.

Ensure that none of the hosing is kinked.

If a particular species or strain of animal is consistently waking up on the anesthesia
machine, an adjustment may need to be made to the procedure for that species to reflect
a higher working concentration of isoflurane/sevoflurane.
Animals are waking up in the chamber:

Ensure that the Chamber Control valve is turned to the Chamber On position and that the
Breather Control valve is turned to the Off position.

If all connections are correct and oxygen is flowing into the system, try increasing the
isoflurane or sevoflurane concentration to 5% for several seconds until the animals appear
to be anesthetized. Then, reduce concentration to the species-appropriate level.
Animals are waking up on the breathing device:

Ensure that the Breather Control valve is turned to the Breather On position and that the
Chamber Control valve is turned to the Off position.

The animal may require a higher concentration of isoflurane or sevoflurane. Try increasing
the concentration on the vaporizer by 0.5%.

If the animal was not properly anesthetized in the chamber or was out of the chamber for
too long prior to moving to the breathing device, the animal may wake up. If this occurs,
place the animal back into the chamber and restart the anesthesia process. Be sure that
the animal is completely anesthetized before removing from the chamber.