UVP GelDoc-Ite Imager - Manual (Page 4)

GelDoc-It
e
Imager 4
Fluorescent Focus Target
Camera and Lens
The GelDoc-It
e
Imager is equipped with a FluorCam 220 camera, a scientific-grade monochrome CCD
camera with a resolution of 1.3 megapixels (1280x1024). The FluorCam 220 utilizes a USB 2.0 PC
connection and is equipped with an 8-48mm optical zoom lens.
All camera settings are factory pre-set for optimum performance when viewing gels, films or
membranes under low light level conditions. Contact UVP Technical Support prior to making any
adjustments to the camera settings.
VisionWorksLS Acquisition and Analysis Software
The GelDoc-It
e
is configured with VisionWorksLS Software for acquisition and analysis of gels, plates
and membranes. The software features image enhancement, complete analysis tools and reporting
capabilities, and is ideal for users who require robust image acquisition and analysis functions.
Ethidium Bromide (EtBr) Emission Filter
The ethidium bromide (50mm square) UV-blocking bandpass interference filter blocks UV and IR
radiation emitted from the transilluminator. The filter is placed in the Filter Tray located beneath the
camera/lens assembly. The filter allows visualization of fluorophores from 580-630nm, targeting the
ethidium bromide emission peak of 605nm.
Additional filters are available for other specific fluorophores, including custom filters. Filters can also be
removed when imaging non-fluorescent media (including protein gels, colony plates, etc.) in order to
produce brighter images. Contact UVP for filter ordering information.
Darkroom
The GelDoc-It
e
Imager’s darkroom features include:

Integrated UV transilluminator (available in 3UV, 2UV and single UV models)

Epi (overhead) LED white light

UV-Blocking Gel Viewer Window built into the darkroom door

Two side doors for easy access to the imaging surface

Single position emission filter tray

UV safety interlock switch to disable UV transillumination when darkroom door is opened
Transilluminator
The GelDoc-It
e
Imager is configured with UVP’s 3UV, 2UV or single UV Benchtop transilluminator
models. Refer to the packing slip for the specific transilluminator model included with your system.
Fluorescent Focus Target
The UVP Fluorescent Focus Target fluoresces when placed
on a UV transilluminator or when exposed to overhead UV.
The Target provides sharp fluorescent images to aid in
adjusting the lens and camera settings for ideal imaging
results.