UVP iBox Spectra Small Animal Imaging System - Manual (Page 11)

iBox Spectra Imaging System 11
Selecting Emission Filters
See the “Installing Emission Filters” section of this manual for instructions on installing individual emission
filters.
Once all filters have been installed, select the Lighting and Filters menu in the TS2 Software and click the
Reset Wheel button under the “Filters” tab to realign and calibrate the filter wheel within the system.
Using the Overhead (Epi) Lighting
To operate the built-in overhead white light, use the Lighting and Filters menu in the TS2 Software and
select White under Epi Illumination.
Using the Warming Plate
NOTE: When the warming plate is plugged into the outlet in the darkroom and the darkroom is turned on,
the warming plate will receive a constant supply of power. The warming plate must be turned off or
unplugged after use, as the software does not control the function of the warming plate.
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Ensure that the warming plate on/off switch is turned on.
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The warming plate will show the temperature of the warming plate in the display. In several
minutes, the warming plate will reach 37°C.
NOTE: The warming plate has an over limit switch that prevents the unit from exceeding 41°C. Should the
unit reach 41°C, the plate must be returned to a temperature of 25°C before the unit reheats.
NOTE: Allow the warming plate to reach room temperature prior to operating. If the unit is extremely cold
when switched on, the warming surface temperature can exceed the preset temperature indicated on the
LCD display.
Using the Sample Viewer Window
The Sample Viewer Window, built into the darkroom door, allows users to view the interior of the darkroom
without opening the darkroom door.
To open the Window, press firmly on the top of the Window cover to release the pressure-sensitive clasp
and open the viewer. The Window glass provides a clear view to the warming plate surface for sample
viewing without opening the darkroom door. In the case that ultraviolet illumination is being used in the
darkroom, the Viewer Window also blocks the user from harmful exposure to UV light.
NOTE: To avoid light leakage, close the Sample Viewer Window prior to capturing a light-sensitive image.
Image Focusing
Prior to capturing an image, prepare the image focus:
1. Turn on the white epi illumination and place the Focus Target (see the “Focus Target” section of
this manual for more information) on the warming plate surface.
2. Using the TS2 Software, click to turn ON the “Live Preview” button. Adjust the exposure, aperture,
zoom (if applicable) and focus controls on the bottom portion of the TS2 Software screen until an
ideal image is visible.
Setting the White Balance
Prior to capturing images using the iBox Spectra, calibrate the system’s white balance. White balance
calibration, in essence, is the process of removing unrealistic color casts so that objects which appear white
in person are rendered white in the captured image. This ensures that the white color in the image is as
close to neutral white as possible and all of the colors in the image also appear true.