UVP Ultraviolet PCR - Manual (Page 10)

Ultraviolet PCR Cabinets and Workstations Page 10
The default timer setting is 30 minutes, adjustable at 5 minute increments from 5 to 60 minutes,
then at 15 minute increments up to 12 hours. To adjust the timer settings:
1. Turn the system on and wait until the timer reads “OFF”.
2. Use a small paper clip to press the Timer calibration button located in the small hole to
the left of the timer for one second.
3. Press the main timer button to adjust the timer settings. Each press will increase the
amount of time by 5 minutes (eg 35:00, 40:00, etc.) until one hour is reached. After one
hour is reached, the time will be adjusted in 15-minute increments and will read without a
colon. For example, one hour will read “1 00,” one hour and 15 minutes will read “1 15”
and so forth.
4. Once the desired time setting is reached, do not press any additional buttons. The timer
will then cycle through the remainder of its settings. When the timer reads “OFF” for 5
seconds without blinking, the timer is ready to use with the new time setting.
Turn the key to the off position after UV irradiation to prevent exposure of samples to the UV.
Ensure the key is completely inserted into the lock.
[Note] Do not attempt to perform PCR procedures with the germicidal lamp on. The germicidal
lamp is used to decontaminate the internal chamber of the between experiments.
[Warning] Exposure to UV light is harmful.
The Makrolon
® door is UV blocking and will not allow UV radiation to pass through the door. The
germicidal lamp will shut off if the panel door is opened. The PCR Workstation contains a powerful
source of UV radiation that will cause damage to unprotected eyes and skin. Before operating any
unit, be sure all personnel in the area are properly protected. Even though the unit shuts the UV off
when the door is open, UV Blocking Eyewear should be worn as well. UVP has a complete line of
UV Blocking Eyewear: Spectacles, Goggles, and Faceshield designed for this purpose.
Extended periods of time UV exposure can be used to disinfect the interior chamber by the
destruction of organisms. Refer to the Maintenance section for information on measuring the
intensity of the UV tubes with a UV meter.
Overhead White Light
The overhead white light can remain lit at any time throughout experiment procedures or the
decontamination process. The fluorescent white light provides a bright light within the PCR
Workstation. To use the white light, press the ON button located on the top panel.
UV Air Circulator
The UV2 PCR Cabinet and UV2 PCR Workstation include
a built-in UV Air Circulator.
To operate the circulator, press the UV/Air Circulator
switch to the ON position. The circulator will pull air from
inside the chamber through the circulator,
decontaminating the air with shortwave UV and blowing
the air back into the chamber.
NOTE: Do not place equipment in front of the air vents to
block air flow.
An optional UV/Air Circulator module can be mounted into
the top of workstation chamber (95-00367-XX only) for
elimination of amplicons of DNA or genomic DNA from a
previously dispensed experiment. The power switch,
outlet, and mounting brackets for the circulator are pre-
installed at the factory if users purchase a module
at a later time.
UV2 PCR Cabinet and Workstation. Airflow
into and out of the UV Air Circulator