Waters Atlantis T3, DC18 and HILIC Silica Columns User Manual

Care and use manual, Atlantis t3, dc, And hilic silica columns

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[ Care and Use ManUal ]

Atlantis Columns

1

I. Int roductIon

Thank you for choosing an Atlantis

®

Column. The manufacture of Atlantis

columns begins with ultrapure reagents to control the chemical composition
and purity of the final product. Atlantis columns are manufactured in a cGMP,
ISO 9001:2000 certified plant with each step being conducted within nar-
row tolerances. Every column is individually tested and Certificates of Batch
Analysis and a Performance Chromatogram are provided with each column.

Waters recommends the use of Sentry

guard columns to maximize column

lifetime and protect the column from contaminants.

contents
I. IntroductIon

II. connectInG tHe coLuMn or cArtrIdGe to tHe

III. systeM ModIfIcAtIon recoMMendAtIons

HPLc systeM

IV. WAters sMALL PArtIcLe sIze (3 µM) coLuMns

– fAst cHroMAtoGrAPHy

V. coLuMn equILIbrAtIon

VI. coLuMn InstALLAtIon Procedure

VII. coLuMn PerforMAnce VALIdAtIon

VIII. InItIAL coLuMn effIcIency deterMInAtIon

IX. coLuMn usAGe

XII. HILIc GettInG stArted

XIII. coLuMn cLeAnInG, reGenerAtInG And storAGe

XIV. troubLesHootInG

AtLAntIs t3, dc

18

And HILIc sILIcA coLuMns

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