2 protecting the separation column, 3 storing the separation column, 4 regenerating the separation column – Metrohm 940 Professional IC Vario ONE/SeS/PP/Prep 3 User Manual

Page 113: Protecting the separation column, Storing the separation column, Regenerating the separation column

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5 Operation and maintenance

940 Professional IC Vario ONE/SeS/PP/Prep 3 (2.940.1530)

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103

5.18.2

Protecting the separation column

We recommend using the following protective measures so that the sepa-
ration column retains its separating efficiency for as long as possible:

Microfilter both the sample and the eluent (0.45 µm filter) and aspirate
the eluent via the aspiration filter (6.2821.090) as well.

Always use a guard column. Information regarding which guard col-
umn is suitable for your separation column can be found in the
Metrohm IC Column Program (which is available from your
Metrohm representative), the leaflet provided along with your separa-
tion column, the product information about the separation column at

http://www.metrohm.com

(Ion Chromatography product area), or

obtained directly from your representative.

Always use the pulsation absorber.

5.18.3

Storing the separation column

Always store separation columns you do not need in a sealed and filled
state according to the column manufacturer's specifications.

5.18.4

Regenerating the separation column

The separation column can be regenerated according to the column man-
ufacturer's specifications if the separation characteristics of the column
have deteriorated. You can find information on regenerating separation
columns available from Metrohm on the leaflet provided with every col-
umn.

NOTE

Regeneration is intended as a last resort. It should not be carried out
regularly.

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