20 initial start-up – Metrohm 940 Professional IC Vario ONE/SeS/PP User Manual

Page 61

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3 Installation

940 Professional IC Vario ONE/SeS/PP (2.940.1500)

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51

After being switched on, the LED on the front of the instrument
flashes. The instrument conducts a system test and establishes a con-
nection to the software. Once the system test is complete and the
connection to the software has been established, the LED lights up
continuously.

3.20

Initial start-up

Even before the guard column and separation column are installed, the
entire system has to be completely rinsed with eluent for the first time.

Rinsing the IC system

CAUTION

The guard column and separation column must not be installed for the
initial start-up.

Make sure that a coupling (6.2744.040) is being used instead of the
columns.

1 Preparing the software

Start the MagIC Net computer program.

Open the Equilibration tab in MagIC Net: Work-
place

Run Equilibration.

Select (or create) a suitable method.
Also see: MagIC Net tutorial and online help.

2 Preparing the instrument

Ensure that the eluent aspiration tubing is immersed in the eluent
and there is enough eluent in the eluent bottle.

STREAM method (recommended): Ensure that the aspiration tub-
ing for the regeneration solution is immersed in the solution and
that there is enough solution; also check that the detector output
capillary is connected to the suppressor's input capillary for rinsing
solution (labeled rinsing solution).
Alternatively, if two bottles are being used for rinsing and regen-
eration: Ensure that the aspiration tubings for the auxiliary solu-
tions (regeneration solution and rinsing solution) are immersed in
the respective solutions and that there is enough solution in both
bottles.

Switch on the instrument.

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