Bio-Rad ReadyPrep™ 2-D Cleanup Kit - Manual (Page 15)

5.2 Preparation of 2-D Rehydration/Sample Buffers
The 2-D rehydration/sample buffer is not a component of
this kit, but is necessary for resuspending the final protein
pellet. It is not provided in the kit because different protein
samples can require different rehydration/sample buffers.
For convenience, a selection of 2-D rehydration/sample
buffer formulas are provided below. The general-purpose
2-D rehydration/sample buffer is also available
preformulated from Bio-Rad (ReadyPrep 2-D starter kit
rehydration/sample buffer, 10 ml, catalog #163-2106).
Users of the ReadyPrep protein extraction kit (membrane I),
catalog #163-2088 or the ReadyPrep protein extraction kit
(cytoplasmic/nuclear), catalog #163-2089 should use the
protein solubilization buffer (PSB) and PSB diluent provided
with these kits to solubilize the protein pellet. PSB is a
proprietary strongly chaotropic 2-D rehydration/sample
buffer (refer to Section 5.2.2.2 for preparation instructions).
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