Bio-Rad ReadyPrep™ 2-D Cleanup Kit - Manual (Page 4)

Section 1
Introduction
The ReadyPrep 2-D cleanup kit facilitates the preparation
of low conductivity samples suitable for isoelectric focusing
(IEF) and 2-D gel electrophoresis. Additionally, the kit
concentrates proteins from samples that are too dilute,
allowing for higher protein loads that can improve spot
detection. The ReadyPrep 2-D cleanup kit can be used
with virtually any protein sample to generate improved 2-D
results by reducing streaking, background staining, and
other gel artifacts associated with substances
contaminating 2D/IEF samples. The entire process can be
completed in approximately one hour and does not
produce alterations in the isoelectric point of proteins. The
procedure works by quantitatively precipitating and
concentrating proteins in a sample while leaving behind
and washing away substances like ionic detergents, salts,
nucleic acids, lipids, and plant-derived phenolic
compounds, all of which are known to interfere with IEF.
The precipitation is not affected by the presence of
detergents, chaotropes or other common buffers, salts and
reagents used to extract or treat proteins. After
precipitation, the proteins are washed and then
resuspended in the IEF/2D-compatible rehydration/sample
buffer of choice, ready for isoelectric focusing or Laemmli
sample buffer, ready for SDS-PAGE.
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