Bio-Rad ReadyPrep™ 2-D Cleanup Kit - Manual (Page 6)

Items Required But Not Provided:
• 1.5 ml microcentrifuge tubes
• Microcentrifuge capable of spinning at 12-16,000 x g at
4°C.
• Rehydration/sample buffer (see Section 5 for recipes and
Section 6 for premixed, ready-to-use rehydration/sample
buffers)
• Vortex mixer
• ReadyPrep proteomic grade water or other ultrapure
water
• RC DC™ Protein Assay (Bio-Rad catalog #500-0121 or
#500-0122)
Section 3
Storage Conditions
Store the kit at room temperature. Wash reagent 2 must be
stored at -20°C for at least 1 hr prior to use. For
convenience, store wash reagent 2 in a -20°C freezer.
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