Bio-Rad ChromLab™ Software - Manual (Page 132)

3 | Preparing the Instrument
130 | NGC Chromatography Systems and ChromLab Software
8. Remove the syringe from the priming port and discard the liquid into waste.
9. Repeat steps 4–8 for the remaining buffer inlet ports.
10. To prime system pump B, select buffer B1 and perform steps 3–9 on system
pump B.
To prime the sample pump
1. Immerse the inlet tubing for the sample pump in the appropriate container.
2. Insert a 30 ml syringe into the priming port.
3. Turn the priming port valve counter-clockwise one full turn to open the seal.
4. Slowly withdraw the plunger to withdraw liquid from the port until air no
longer enters the syringe (approximately 10 ml) and all tubing is filled with
liquid.
5. Turn the priming port valve clockwise to close the port.
6. Remove the syringe from the priming port and discard the liquid into waste.